Nanog antibody [N3C3]
|Applications||ELISA, ICC/IF, IHC, IHC-Fr, IP, WB|
Not tested in other applications.
| Immunohistochemistry||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||35 kDa (note)
|Positive Controls||HeLa nucleus , NIH-3T3 , mouse ESC|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||Nanog homeobox|
|Product Description||Rabbit Polyclonal antibody to Nanog (Nanog homeobox)|
|Background||Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.|
|Synonyms||NANOG antibody, homeobox transcription factor Nanog-delta 48 antibody, hNanog antibody, homeobox protein NANOG antibody, homeobox transcription factor Nanog antibody, Nanog homeobox antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human NANOG. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Chimpanzee (98%)|