NeuN antibody [2Q158]
|Applications||ICC/IF, IHC-Fr, IHC-P, WB|
|Application Note||Recommended Starting Dilutions:|
For ICC: Use at a dilution of 1:5-1:50 (permeabilize neurons in cell culture).
For IHC-P: Use at a dilution of 1:50. Works best on polyester wax embedded tissue.
For WB: 1:500-1:3000. Recognizes 2-3 bands in the 46-48kD range and possibly another band at approximately 66kD.
Optimal dilutions/concentrations should be determined by the end user
|Positive Controls||IMR32 , IMR32 nuclear extract|
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A affinity purified|
|Full Name||RNA binding protein, fox-1 homolog (C. elegans) 3|
|Product Description||Mouse monoclonal antibody to NeuN|
|Background||Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei) is an excellent marker for neurons in primary cultures and in retinoic acid-stimulated P19 cells. It is also useful for identifying neurons in transplants. NeuN (Neuronal Nuclei) is a neuron-specific, DNA-binding nuclear protein in vertebrates. In mice, NeuN is observed in most neuronal cell types throughout the nervous system, including cerebellum, cerebral cortex, hippocampus, thalamus, and spinal cord, as well as the dorsal root ganglia, sympathetic chain ganglia, and enteric ganglia of the peripheral nervous system. NeuN immunoreactivity is first observed in neurons when they become post-mitotic and are initiating cellular and morphological differentiation. No staining is observed in proliferative zones. NeuN has been used as an immunohistochemical marker for excitotoxic lesions of the brain, as well as in the diagnosis of a wide range of human tissue specimens from the central and peripheral nervous systems.|
|Synonyms||NeuN, RNA binding protein, fox1 homolog (C. elegans) 3, HRNBP3, fox1 homolog C, FLJ58356, hexaribonucleotide binding protein3, hexaribonucleotide binding protein 3, neuronal nuclei, OTTHUMP00000196558, FOX-3, FLJ56884, fox-1 homolog C, Neuron Specific Nuclear Protein (NeuN), FOX3, Neu, D11Bwg0517e, FOX 3, fox 1 homolog C|
|Specificity||Vertebrate neuron-specific nuclear protein called NeuN (Neuronal Nuclei). Recognizes 2-3 bands in the 46-48kD range and possibly another band at approximately 66kD |
|Immunogen||Purified cell nuclei from mouse brain|
|Species Reactivity||Human, Mouse, Rat, Ferret, Salamander|