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1 .GTX114593 Nucleobindin 1 antibody [N3C3]

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Nucleobindin 1 antibody [N3C3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse
  • ★★★★☆   (  1  ) Publication  ( 3 )
Cat No: GTX114593
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
  • Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
  • Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
  • Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
  • Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX114593
  • Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX114593
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  • Datasheet
  • Star Review (1)
    Reference (3)
  • Bio-info
  • Protocol & MSDS
  • Application Information: Nucleobindin 1 antibody [N3C3]

Catalog Number GTX114593
★★★★☆
( 1 )
Publication ( 3 )
Product Name Nucleobindin 1 antibody [N3C3]
See all Nucleobindin 1 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size54 kDa (note) IsoForm (2)
Positive ControlsA549 , HeLa , HepG2 , mouse brain
Form SuppliedLiquid
Concentration0.94 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Nucleobindin 1 antibody [N3C3]

Full Namenucleobindin 1
BackgroundThis gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq]
SynonymsCALNUC antibody, DKFZp686A15286 antibody, FLJ40471 antibody, NUC antibody, NUCB1 antibody, nucleobindin-1 antibody, nucleobindin 1 antibody
Cellular LocalizationGolgi apparatus , cis-Golgi network membrane; Peripheral membrane protein; Lumenal side (By similarity) , Cytoplasm (By similarity)
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Nucleobindin 1. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Pig, Bovine
Predict Reactivity NoteRat (85%), Pig (91%), Bovine (91%)
ConjugationUnconjugated

  • Storage Conditions: Nucleobindin 1 antibody [N3C3]

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Nucleobindin 1 antibody [N3C3] validated data
  • Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
    • GTX114593 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using Nucleobindin 1(GTX114593) antibody at 1:200 dilution.
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
    • GTX114593 WB Image
      Sample (50 ug of whole cell lysate)
      A: Mouse brain
      10% SDS PAGE
      GTX114593 diluted at 1:1000
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
    • GTX114593 WB Image
      Sample (30 ug of whole cell lysate)
      A: Hela
      10% SDS PAGE
      GTX114593 diluted at 1:1000
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
    • GTX114593 ICC/IF Image
      Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.
      Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody [N3C3] (GTX114593) diluted at 1:500.
      Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX114593
    • GTX114593 IHC-P Image
      Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX114593
    • GTX114593 IHC-P Image
      Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse brain stem by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse brain stem.
      Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
    • GTX114593 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using Nucleobindin 1(GTX114593) antibody at 1:200 dilution.
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
    • GTX114593 WB Image
      Sample (50 ug of whole cell lysate)
      A: Mouse brain
      10% SDS PAGE
      GTX114593 diluted at 1:1000
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in Western Blot (WB). GTX114593
    • GTX114593 WB Image
      Sample (30 ug of whole cell lysate)
      A: Hela
      10% SDS PAGE
      GTX114593 diluted at 1:1000
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114593
    • GTX114593 ICC/IF Image
      Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at Golgi apparatus by immunofluorescent analysis.
      Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: Nucleobindin 1 protein stained by Nucleobindin 1 antibody [N3C3] (GTX114593) diluted at 1:500.
      Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX114593
    • GTX114593 IHC-P Image
      Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.
      Top
  • Anti-Nucleobindin 1 antibody [N3C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX114593
    • GTX114593 IHC-P Image
      Nucleobindin 1 antibody [N3C3] detects Nucleobindin 1 protein at cytosol on mouse brain stem by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse brain stem.
      Nucleobindin 1 antibody [N3C3] (GTX114593) dilution: 1:500.
      Top
  • StarReview
  • Nucleobindin 1 antibody [N3C3]

  • Rating : ★★★★☆ ( Average 4.0 based on 1 users reviews)
  • Immunocytochemistry/ Immunofluorescence (ICC/IF)(1) :★★★★☆
  • Anonymous StarReview submitted on 21 Dec 2015
  • Score : ★★★★☆
  • Application Tested : ICC/IF
  • Sample Species : Human
  • Sample : RPE cells
  • Blocking conditions
  • Blocking Buffer : 0.2% BSA, 0.1% saponin in PBS
  • Incubation Time & Temperature : RT (℃)/30min
  • Primary antibody
  • Dilution : 1:100( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
Back to Top
  • Reference for Nucleobindin 1 antibody [N3C3]
Application References Application Reactivity
May EW et al. Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: Reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance. Pharmacol Res 90C:1-17 (2014). WB Human
Huang HJ et al. Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells. Mol Biosyst (Epub ahead of print) (2014). WB Human
May EW et al. Identification of up- and down-regulated proteins in doxorubicin-resistant uterine cancer cells: Reticulocalbin-1 plays a key role in the development of doxorubicin-associated resistance. Pharmacol Res (Epub ahead of print) (2014). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensNUCB14924Q02818, B2RD64601323BC002356AAH0235653.89
Homo sapiensNUCB14924Q02818, B2RD64601323NM_006184NP_00617553.89

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusNucb118220Q02819NM_008749NP_03277553.01
Mus musculusNucb118220Q02819NM_001163662NP_00115713453.42
Rattus norvegicusNucb184595Q63083NM_053463NP_44591553.52
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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