OPG antibody [bony-1]

Cat No:

GTX18051

Package	Price	Qty
50 μg	USD 319.00

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Catalog Number	GTX18051 ☆☆☆☆☆ ( 0 ) ( 0 )
Product Name	OPG antibody [bony-1] See all Osteoprotegerin products
Applications	FACS, IP
Application Note	FACS: 1/1000. IP: 1/200. Optimal dilutions/concentrations should be determined by the end user.
Concentration	1 mg/ml (Please refer to the vial label for the specific concentration)
Purification	>95% by SDS-PAGE

Background	Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined.
Cellular Localization	Secreted
Specificity	Recognizes human OPG
Host	Rat
Clonality	Monoclonal
Clone Name	bony-1
Isotype	IgG2a
Immunogen	Recombinant fragment, corresponding to amino acids 22-202 of Human Osteoprotegerin.
Antigen Species	Human
Species Reactivity	Human
Conjugation	Unconjugated

Storage Buffer	Preservative: 0.02% Sodium Azide. Constituents: PBS
Storage Instruction	Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

OPG antibody [bony-1] validated data

GTX18051 FACS Image

FACS analysis of cells with MAb to OPG (human) (GTX18051). HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to OPG (human) (Bony-1) (GTX18051). After washing in FACS buffer, RPE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

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GTX18051 FACS Image

FACS analysis of cells with MAb to OPG (human) (GTX18051). HEK 293T cells (5x105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to OPG (human) (Bony-1) (GTX18051). After washing in FACS buffer, RPE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

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Primary Antibodies