ORP150 antibody [C2C3], C-term
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||111 kDa (note)
|Positive Controls||MCF-7 , MDA-MB-231 , mouse liver , rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||hypoxia up-regulated 1|
|Product Description||Rabbit Polyclonal antibody to ORP150 (hypoxia up-regulated 1)|
|Background||The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. [provided by RefSeq]|
|Synonyms||DKFZp686N08236 antibody, FLJ94899 antibody, FLJ97572 antibody, Grp170 antibody, HSP12A antibody, ORP150 antibody, HYOU1 antibody, hypoxia up-regulated protein 1 antibody, glucose-regulated protein 170 antibody, ORP-150 antibody, 150 kDa oxygen-regulated protein antibody, 170 kDa glucose-regulated protein antibody, GRP-170 antibody, oxygen regulated protein (150kD) antibody, hypoxia up-regulated 1 antibody|
|Cellular Localization||Endoplasmic reticulum lumen|
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human ORP150. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Bovine (88%)|