|Applications||FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB|
Not tested in other applications.
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||39 kDa (note)
|Positive Controls||hESC , mouse ESC , NT2D1|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||POU class 5 homeobox 1|
|Product Description||Rabbit Polyclonal antibody to OCT4 (POU class 5 homeobox 1)|
|Background||This gene encodes a transcription factor containing a POU homeodomain. This transcription factor plays a role in embryonic development, especially during early embryogenesis, and it is necessary for embryonic stem cell pluripotency. A translocation of this gene with the Ewing's sarcoma gene, t(6;22)(p21;q12), has been linked to tumor formation. Alternative splicing, as well as usage of alternative translation initiation codons, results in multiple isoforms, one of which initiates at a non-AUG (CUG) start codon. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq]|
|Synonyms||MGC22487 antibody, OTF-3 antibody, OTF3 antibody, OTF4 antibody, POU5F1 antibody, POU-type homeodomain-containing DNA-binding protein antibody, octamer-binding protein 3 antibody, POU domain transcription factor OCT4 antibody, octamer-binding protein 4 antibody, POU domain, class 5, transcription factor 1 antibody, octamer-binding transcription factor 3 antibody, octamer-binding transcription factor-3 antibody, POU class 5 homeobox 1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Cat, Pig, Rabbit, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Rat (83%), Cat (94%), Pig (93%), Rabbit (90%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (91%)|