GeneTex
  • Country / Location Selection

United States (US)

OneStep Polymer HRP anti-mouse/rat/rabbit (Ready-to-Use)

OneStep Polymer HRP anti-mouse/rat/rabbit (Ready-to-Use). GTX83398

Cat. No. GTX83398

Application

WB, IHC

Reactivity

Mouse, Rat, Rabbit
Package
100 test ($199)
PRODUCT

Summary

This kit employs Polymer technology shown to provide increased sensitivity and detection. It is most sensitive IHC kit and provides the user with a rapid and easy to use IHC detection system. (Reagent : 10 ml = 100 tests, when 0.1 ml is applied per slide)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Target Isotype

IgG

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_11162022

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

antimouse HRP polymer , anti-rat HRP polymer , anti-rabbit HRP polymer , anti-mouse HRP polymer , IHC Polymer , antirabbit HRP polymer , antirat HRP polymer , anti rat HRP polymer , anti mouse HRP polymer , anti rabbit HRP polymer

Background

Immunohistochemistry (IHC) is the localization of antigens in tissue sections/cells using antibodies as specific reagents. Visualization of antibody signal is often significantly improved using the biotin-avidin amplification system. This system, however, adds significant time to experimental protocols and results in higher background due to the presence of endogenous biotin in tissue.

Kit Components:
polymer 100 ml (The polymer is composed of secondary antibodies (several units)+ HRP (several units))


GeneTex’s One-Step anti-mouse/rat/rabbit IgG (H+L) uses the power of next-generation Polymer technology to provide dramatic signal amplification without the need for biotin. This reagent has been shown to provide increased sensitivity and detection, while reducing the incubation time and required quantity of primary antibody. This reagent can be used to enhance staining of membranes, cytoplasmic and nuclear antigens. It provides the user with a rapid and easy to use IHC detection system.


Features and Benefits
‧ Lower background: Amplification without biotin avoids background from endogenous biotin
‧ Higher sensitivity than standard IHC detection
‧ Faster: Staining time reduced to 15 minutes!
‧ Compatible with either manual or automated systems
‧ Better results on difficult-to-work antibodies and nuclear staining
‧ Save cost due to further dilution of primary antibody

DATA IMAGES

OneStep Polymer HRP anti-mouse/rat/rabbit (Ready-to-Use). GTX83398

GTX83398 IHC Image

Human tonsil tissue sections stain with mouse anti-Ki67 primary antibody and detected using GeneTex
OneStep Polymer (incubation time, 15 minutes at RT).

REFERENCE

REVIEW

There are currently no reviews for OneStep Polymer HRP anti-mouse/rat/rabbit (Ready-to-Use) (GTX83398). Be the first to share your experience with this product.
Package List Price ($)
$ 199