|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||66 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Product Description||Rabbit Polyclonal antibody to Optineurin (optineurin)|
|Background||This gene encodes the coiled-coil containing protein optineurin. Optineurin may play a role in normal-tension glaucoma and adult-onset primary open angle glaucoma. Optineurin interacts with adenovirus E3-14.7K protein and may utilize tumor necrosis factor-alpha or Fas-ligand pathways to mediate apoptosis, inflammation or vasoconstriction. Optineurin may also function in cellular morphogenesis and membrane trafficking, vesicle trafficking, and transcription activation through its interactions with the RAB8, huntingtin, and transcription factor IIIA proteins. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]|
|Synonyms||ALS12 antibody, FIP2 antibody, GLC1E antibody, HIP7 antibody, HYPL antibody, NRP antibody, TFIIIA-INTP antibody, OPTN antibody, HIP-7 antibody, optic neuropathy-inducing protein antibody, tumor necrosis factor alpha-inducible cellular protein containing leucine zipper domains antibody, huntingtin yeast partner L antibody, transcrption factor IIIA-interacting protein antibody, Huntingtin interacting protein L antibody, transcription factor IIIA-interacting protein antibody, E3-14.7K-interacting protein antibody, FIP-2 antibody, nemo-related protein antibody, huntingtin-interacting protein L antibody, huntingtin-interacting protein 7 antibody, optineurin antibody|
|Cellular Localization||Cytoplasm , perinuclear region , Golgi apparatus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Optineurin. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Mouse (82%), Rat (83%), Pig (85%), Rhesus Monkey (96%), Bovine (86%)|