P Glycoprotein antibody [UIC2]
|Applications||FACS, ICC/IF, IHC-Fr, IHC-P, IP|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
| IHC (Formalin-fixed paraffin-embedded sections)||Assay-dependent dilution
| Immunoprecipitation||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein A purified|
|Purification Note||From tissue culture supernatant|
|Full Name||ATP binding cassette subfamily B member 1|
|Product Description||Mouse monoclonal antibody [UIC2] to P Glycoprotein|
|Background||The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq, Jul 2008]|
|Synonyms||ABCB1 Antibody , PGY1 Antibody , GP170 Antibody , ABC20 Antibody , CD243 Antibody , P-GP Antibody , ATP-binding cassette, sub-family B (MDR/TAP), member 1 Antibody , MDR1 Antibody , CLCS Antibody|
|Specificity||This antibody recognizes an extracellular conformational epitope of CD243, also known as MDR1 (multi-drug resistance protein 1) and Pgp (P-glycoprotein).|
|Immunogen||Mouse Balb/c 3T3 fibroblasts transfected with human P Glycoprotein cDNA.|