|Applications||ICC/IF, IHC-Fr, IHC-P, WB|
Not tested in other applications.
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||33 kDa (note)
|Positive Controls||Jurkat , Raji , mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||poly(A) binding protein, nuclear 1|
|Product Description||Rabbit Polyclonal antibody to PABPN1 (poly(A) binding protein, nuclear 1)|
|Background||This gene encodes an abundant nuclear protein that binds with high affinity to nascent poly(A) tails. The protein is required for progressive and efficient polymerization of poly(A) tails on the 3' ends of eukaryotic genes and controls the size of the poly(A) tail to about 250 nt. At steady-state, this protein is localized in the nucleus whereas a different poly(A) binding protein is localized in the cytoplasm. An expansion of the trinucleotide (GCG) repeat from normal 6 to 8-13 at the 5' end of the coding region of this gene leads to autosomal dominant oculopharyngeal muscular dystrophy (OPMD) disease. Multiple splice variants have been described but their full-length nature is not known. One splice variant includes introns 1 and 6 but no protein is formed. [provided by RefSeq]|
|Synonyms||OPMD antibody, PAB2 antibody, PABII antibody, PABP-2 antibody, PABP2 antibody, PABPN1 antibody, poly(A) binding protein II antibody, polyadenylate-binding protein 2 antibody, poly(A) binding protein 2 antibody, "poly(A) binding protein, nuclear 1 antibody"|
|Cellular Localization||Nucleus (By similarity) , Cytoplasm (By similarity) |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PABPN1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Dog, Pig, Bovine|
|Predict Reactivity Note||Rat (99%), Zebrafish (86%), Dog (100%), Pig (100%), Bovine (100%)|