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PAD4 antibody

Anti-PAD4 antibody used in Western Blot (WB). GTX54621
Anti-PAD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54621

Cat. No. GTX54621

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all PAD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human PADI4 (NP_036519.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peptidyl arginine deiminase 4 , PAD , PAD4 , PADI5 , PDI4 , PDI5

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PAD4 antibody used in Western Blot (WB). GTX54621

GTX54621 WB Image

WB analysis of various sample lysates using GTX54621 PAD4 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PAD4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54621

GTX54621 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54621 PAD4 antibody.
Blue : DAPI

REFERENCE

Application Reference

Kim SW et al. Int J Mol Sci 2020; 21 (20) Adenosine Triphosphate Accumulated Following Cerebral Ischemia Induces Neutrophil Extracellular Trap Formation.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399