PATJ antibody [N1], N-term

Host / ClonalityRabbit Polyclonal
ApplicationsIHC-P, WB
Species reactivityHuman, Mouse
( 3 )

Cat No:

GTX104753

Package	Price	Qty
25 μl	USD 169.00
100 μl	USD 319.00

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Protocol & MSDS

Application Information: PATJ antibody [N1], N-term

Catalog Number

GTX104753

Product Name

PATJ antibody [N1], N-term

See all PATJ products

Applications

IHC-P, WB

Application Note

	Suggested dilution	Reference
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Western Blot (WB)	1:500-1:3000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

196 kDa (note) IsoForm

(2)

Positive Controls

Mouse bladder

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: PATJ antibody [N1], N-term

Full Name	InaD-like (Drosophila)
Background	This gene encodes a protein with multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. This protein localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors. [provided by RefSeq]
Synonyms	Cipp antibody, FLJ26982 antibody, InaD-like antibody, PATJ antibody, hINADL antibody, INADL antibody, protein associated to tight junctions antibody, PDZ domain protein antibody, inaD-like protein antibody, channel-interacting PDZ domain protein antibody, PALS1-associated tight junction protein antibody, inactivation no after-potential D-like protein antibody, InaD-like (Drosophila) antibody
Cellular Localization	Membrane , Cell junction , tight junction , Apical cell membrane
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PATJ. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Bovine
Predict Reactivity Note	Bovine (100%)
Conjugation	Unconjugated

Storage Conditions: PATJ antibody [N1], N-term

Storage Buffer	1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

PATJ antibody [N1], N-term validated data

GTX104753 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

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GTX104753 IHC-P Image

PATJ antibody [N1], N-term detects PATJ protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

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GTX104753 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

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GTX104753 IHC-P Image

PATJ antibody [N1], N-term detects PATJ protein at cytoplasm in human colon by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
PATJ antibody [N1], N-term (GTX104753) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Top

Reference for PATJ antibody [N1], N-term

Application References	Application	Reactivity
Gagnoux-Palacios L et al. Cell polarity and adherens junction formation inhibit epithelial Fas cell death receptor signaling. J Cell Biol (Epub ahead of print) (2018).	WB	Human
Jiang X et al. Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development. Gene Expr Patterns 27:93-98 (2017).
Lennon FE et al. Transactivation of the receptor-tyrosine kinase ephrin receptor A2 is required for the low molecular weight hyaluronan-mediated angiogenesis that is implicated in tumor progression. J Biol Chem 289 (35):24043-58 (2014).	WB	Human

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	INADL	10207	Q8NI35	603199		NP_001337074	204.75
Homo sapiens	INADL	10207	Q8NI35	603199	NM_176877	NP_795352	196.42

*MSDS

MSDS-Glycerol.pdf

MSDS-PBS.pdf

MSDS-Thimerosal.pdf

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PATJ antibody [N1], N-term

PATJ antibody [N1], N-term

Application Information: PATJ antibody [N1], N-term

Specifications: PATJ antibody [N1], N-term

Storage Conditions: PATJ antibody [N1], N-term