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Catalog Number
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GTX113241
(
4
)
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|
Product Name
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PAX6 antibody
|
| Applications | ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Application Note |
| Suggested dilution | Reference |
| Immunocytochemistry/ Immunofluorescence (ICC/IF) | 1:100-1:1000* |
Y |
| IHC (Frozen sections) | 1:100-1:1000* |
|
| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
|
| Immunoprecipitation | 1:500-1:1000* |
|
| Western Blot (WB) | 1:500-1:3000* |
Y |
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|
| Predicted Target Size | 47 kDa (note)
 (10)
|
| Positive Controls | 293T , HeLa , mouse brain , rat brain |
| Form Supplied | Liquid |
| Concentration | 1.8
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Purified by antigen-affinity chromatography. |
| Full Name | paired box 6 |
| Background | This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq] |
| Synonyms | AN antibody, AN2 antibody, D11S812E antibody, MGC17209 antibody, MGDA antibody, WAGR antibody, PAX6 antibody, paired box protein Pax-6 antibody, aniridia type II protein antibody, oculorhombin antibody, paired box homeotic gene-6 antibody, paired box 6 antibody |
| Cellular Localization | Nucleus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAX6. The exact sequence is proprietary. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Rat |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, Bovine |
| Predict Reactivity Note | Xenopus laevis (94%), Dog (100%), Pig (100%), Rabbit (99%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%) |
| Conjugation | Unconjugated |