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1 .GTX113241 PAX6 antibody

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PAX6 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-Fr, IHC-P, IP, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 4 )
Cat No: GTX113241
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-PAX6 antibody used in Immunoprecipitation. GTX113241
  • Anti-PAX6 antibody used in IHC (Frozen sections). GTX113241
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
  • Anti-PAX6 antibody used in IHC (Frozen sections). GTX113241
  • Anti-PAX6 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX113241
  • Anti-PAX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113241
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
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  • Datasheet
  • Star Review (0)
    Reference (4)
  • Bio-info
  • Protocol & MSDS
  • Application Information: PAX6 antibody

Catalog Number GTX113241
☆☆☆☆☆
( 0 )
Publication ( 4 )
Product Name PAX6 antibody
See all PAX6 products
ApplicationsICC/IF, IHC-Fr, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000* Y
IHC (Frozen sections)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:500-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size47 kDa (note) IsoForm (10)
Positive Controls293T , HeLa , mouse brain , rat brain
Form SuppliedLiquid
Concentration1.8 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: PAX6 antibody

Full Namepaired box 6
BackgroundThis gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq]
SynonymsAN antibody, AN2 antibody, D11S812E antibody, MGC17209 antibody, MGDA antibody, WAGR antibody, PAX6 antibody, paired box protein Pax-6 antibody, aniridia type II protein antibody, oculorhombin antibody, paired box homeotic gene-6 antibody, paired box 6 antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human PAX6. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteXenopus laevis (94%), Dog (100%), Pig (100%), Rabbit (99%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: PAX6 antibody

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Disease Related > Diabetes
  • Immunology > Autoimmunity > Autoimmune diseases > Diabetes
  • Neuroscience > Cell type markers > Neuronal markers
  • Neuroscience > Sensory > Visual
More Hide
PAX6 antibody validated data
  • Anti-PAX6 antibody used in Immunoprecipitation. GTX113241
    • GTX113241 IP Image
      PAX6 antibody immunoprecipitates PAX6 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 μg of PAX6 antibody (GTX113241) 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody (GTX113241) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-PAX6 antibody used in IHC (Frozen sections). GTX113241
    • GTX113241 IHC-Fr Image
      PAX6 antibody detects PAX6 protein expression by immunohistochemical analysis.
      Sample: Frozen sectioned E13.5 Rat brain.
      Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
      Blue: Fluoroshield with DAPI (GTX30920).
      Top
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
    • GTX113241 WB Image
      PAX6 antibody detects PAX6 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted by 1:1000.
      Top
  • Anti-PAX6 antibody used in IHC (Frozen sections). GTX113241
    • GTX113241 IHC-Fr Image
      PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis.
      Sample: Frozen section of embryonic mouse brain (mE12.5).
      PAX6 antibody (GTX113241) diluted at 1:250.
      Top
  • Anti-PAX6 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX113241
    • GTX113241 IHC-P Image
      PAX6 antibody detects PAX6 protein by immunohistochemical analysis.
      Samples: Paraffin-Embedded mouse retina.
      Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:250.
      Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
      Blue: Fluoroshield with DAPI (GTX30920).
      Top
  • Anti-PAX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113241
    • GTX113241 ICC/IF Image
      PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.
      Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:500.
      Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
      Scale bar = 10 μm.
      Top
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
    • GTX113241 WB Image
      Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
    • GTX113241 WB Image
      Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-PAX6 antibody used in Immunoprecipitation. GTX113241
    • GTX113241 IP Image
      PAX6 antibody immunoprecipitates PAX6 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX6 protein by 2 μg of PAX6 antibody (GTX113241) 10% SDS-PAGE The immunoprecipitated PAX6 protein was detected by PAX6 antibody (GTX113241) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-PAX6 antibody used in IHC (Frozen sections). GTX113241
    • GTX113241 IHC-Fr Image
      PAX6 antibody detects PAX6 protein expression by immunohistochemical analysis.
      Sample: Frozen sectioned E13.5 Rat brain.
      Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
      Blue: Fluoroshield with DAPI (GTX30920).
      Top
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
    • GTX113241 WB Image
      PAX6 antibody detects PAX6 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted by 1:1000.
      Top
  • Anti-PAX6 antibody used in IHC (Frozen sections). GTX113241
    • GTX113241 IHC-Fr Image
      PAX6 antibody detects PAX6 protein at nucleus in embryonic mouse brain by immunohistochemical analysis.
      Sample: Frozen section of embryonic mouse brain (mE12.5).
      PAX6 antibody (GTX113241) diluted at 1:250.
      Top
  • Anti-PAX6 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX113241
    • GTX113241 IHC-P Image
      PAX6 antibody detects PAX6 protein by immunohistochemical analysis.
      Samples: Paraffin-Embedded mouse retina.
      Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:250.
      Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
      Blue: Fluoroshield with DAPI (GTX30920).
      Top
  • Anti-PAX6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113241
    • GTX113241 ICC/IF Image
      PAX6 antibody detects PAX6 protein at nucleus by immunofluorescent analysis.
      Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: PAX6 protein stained by PAX6 antibody (GTX113241) diluted at 1:500.
      Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
      Scale bar = 10 μm.
      Top
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
    • GTX113241 WB Image
      Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-PAX6 antibody used in Western Blot (WB). GTX113241
    • GTX113241 WB Image
      Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PAX6 antibody (GTX113241) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
Back to Top
  • Reference for PAX6 antibody
Application References Application Reactivity
Zhang Q et al. Neural Progenitor-Like Cells Induced From Human Gingiva-Derived Mesenchymal Stem Cells Regulate Myelination of Schwann Cells in Rat Sciatic Nerve Regeneration. Stem Cells Transl Med (2016). ICC/IF Human
Chen C et al. Role of astroglia in Down's syndrome revealed by patient-derived human-induced pluripotent stem cells. Nat Commun 5:4430 (2014). ICC/IF Human
Chang WF et al. SMN is required for the maintenance of embryonic stem cells and neuronal differentiation in mice. Brain Struct Funct (2014). WB Mouse
Chen KC et al. MicroRNA-328 may influence myopia development by mediating the PAX6 gene. Invest Ophthalmol Vis Sci 53 (6):2732-9 (2012).
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NP_00124539148.23
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NP_00124539248.23
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NP_00129708748.23
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NP_00129708844.32
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108BC011953AAH1195346.69
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NM_000280NP_00027146.69
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NM_001127612NP_00112108446.69
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NM_001258464NP_00124539346.69
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NM_001258465NP_00124539446.69
Homo sapiensPAX65080P26367, Q66SS1106210, 120200, 120430, 129750, 136520, 148190, 165550, 206700, 604219, 604229, 607108NM_001604NP_00159548.23

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusPax618508A2A410NM_001244202NP_00123113146.69
Mus musculusPax618508A2A410NP_00123112748.23
Mus musculusPax618508A2A410NP_00129707346.69
Rattus norvegicusPax625509P63016NM_013001NP_03713346.77
Mus musculusPax618508A2A410NM_001244201NP_00123113046.69
Mus musculusPax618508A2A410NP_00123112948.23
Mus musculusPax618508A2A410NM_013627NP_03865548.23
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf
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