Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||71 kDa (note)
|Positive Controls||293T , HeLa , HepG2 , Neuro2A , GL261 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||phosphoenolpyruvate carboxykinase 2 (mitochondrial)|
|Product Description||Rabbit Polyclonal antibody to PCK2 (phosphoenolpyruvate carboxykinase 2 (mitochondrial))|
|Background||This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq]|
|Synonyms||PEPCK antibody, PEPCK-M antibody, PEPCK2 antibody, PCK2 antibody, phosphoenolpyruvate carboxylase antibody, PEP carboxykinase antibody, phosphopyruvate carboxylase antibody, phosphoenolpyruvate carboxykinase [GTP], mitochondrial antibody, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody|
|Cellular Localization||Mitochondrion |
|Immunogen||Recombinant protein encompassing a sequence within the N-terminus region of human PCK2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Pig (96%), Rhesus Monkey (98%), Bovine (96%)|