|Applications||ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB|
Not tested in other applications.
| Immunohistochemistry||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||29 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , NIH-3T3 , PC-12 , *A549 , *SiHa|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||proliferating cell nuclear antigen|
|Product Description||Rabbit Polyclonal antibody to PCNA (proliferating cell nuclear antigen)|
|Background||The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq]|
|Synonyms||MGC8367 antibody, PCNA antibody, cyclin antibody, DNA polymerase delta auxiliary protein antibody, proliferating cell nuclear antigen antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PCNA. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Zebrafish (92%), Xenopus laevis (88%), Pig (99%), Chicken (95%), Rhesus Monkey (100%), Bovine (98%)|