- Host / ClonalityRabbit Polyclonal
PDEF antibody validated data
|Full Name||SAM pointed domain containing ets transcription factor|
|Synonyms||PDEF antibody, RP11-375E1__A.3 antibody, bA375E1.3 antibody, SPDEF antibody, prostate epithelium-specific Ets transcription factor antibody, prostate-specific Ets antibody, SAM pointed domain-containing Ets transcription factor antibody, prostate-derived Ets factor antibody, SAM pointed domain containing ets transcription factor antibody|
|Product Description||Rabbit Polyclonal antibody to PDEF (SAM pointed domain containing ets transcription factor)|
|Background||PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.[supplied by OMIM]|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 79 and 335 of PDEF (Uniprot ID#O95238)|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Human (100%)|
|Application Note||Antibody reactive against recombinant protein, detects immunogen. Further validation in progress.|
|Positive Controls||Target recombinant protein|
|Predicted Target Size||38 kDa (note)|
|Purification||Purified by antigen-affinity chromatography.|
|Storage Buffer||1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.|
|Storage Instruction||Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
Why is the observed Western Blot band size different from predicted size?
The predicted M.W. is based on protein sequence analysis; however, some factors might lead to an observed band size that is different from the predicted size. The reasons might include:
1.Post-translational modification (PTM):
a. Some post-translational modifications might lead to increased protein size, including
phosphorylation, acetylation, methylation, glycosylation, sumoylation, ubiquitination,
b. Some post-translational modifications might lead to decreased protein size including
phosphatidylethanolamine conjunction (e.g. LC3-II)
c. Some proteins may be cleaved to form an active or mature form; this process will
lead to a decreased protein size (e.g. Notch activation, Caspase activation, etc.)
d. Some websites provide useful PTM information
iv.CBS data sets http://www.cbs.dtu.dk/databases/
v.CBS prediction Servers http://www.cbs.dtu.dk/services/
2.mRNA splice variants (Isoforms):
Through alternative splicing, one gene can generate different proteins with different M.W. Regulation of alternative splicing depends upon cell type, conditions, etc.
Some proteins could form dimers or multimers, increasing the M.W. This phenomenon usually can be found in reducing gel condition; however, strong interactions may still be seen with higher molecular weight proteins even in denaturing gel.
The observed size could also potentially be influenced by the protein charge
Different species likely have different protein sequence and PTM, which can lead to a different protein M.W.
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