PDI antibody [N1N3]
|Applications||EM, ICC/IF, IHC-P, WB|
Not tested in other applications.
| Electron microscopy||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||57 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||prolyl 4-hydroxylase, beta polypeptide|
|Product Description||Rabbit Polyclonal antibody to PDI (prolyl 4-hydroxylase, beta polypeptide)|
|Background||This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. [provided by RefSeq]|
|Synonyms||DSI antibody, ERBA2L antibody, GIT antibody, P4Hbeta antibody, PDI antibody, PDIA1 antibody, PHDB antibody, PO4DB antibody, PO4HB antibody, PROHB antibody, P4HB antibody, cellular thyroid hormone-binding protein antibody, protein disulfide-isomerase antibody, "protein disulfide isomerase family A, member 1 antibody", glutathione-insulin transhydrogenase antibody, protein disulfide isomerase/oxidoreductase antibody, "procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide antibody", collagen prolyl 4-hydroxylase beta antibody, protocollagen hydroxylase antibody, prolyl 4-hydroxylase subunit beta antibody, thyroid hormone-binding protein p55 antibody, protein disulfide isomerase-associated 1 antibody, p55 antibody, "prolyl 4-hydroxylase, beta polypeptide antibody"|
|Cellular Localization||Endoplasmic reticulum lumen , Melanosome , Cell membrane; Peripheral membrane protein (Potential) |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PDI. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Rabbit, Chicken, Chimpanzee, Bovine|
|Predict Reactivity Note||Rat (92%), Rabbit (91%), Chicken (83%), Chimpanzee (100%), Bovine (93%)|