PFK (muscle) antibody [C1C3]
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||85 kDa (note)
|Positive Controls||293T , A431 , H1299 , Molt-4 , Raji , mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||phosphofructokinase, muscle|
|Product Description||Rabbit Polyclonal antibody to PFK (muscle) (phosphofructokinase, muscle)|
|Background||The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 184.108.40.206). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]|
|Synonyms||GSD7 antibody, MGC8699 antibody, PFK-1 antibody, PFK1 antibody, PFKA antibody, PFKX antibody, PFKM antibody, phosphohexokinase antibody, phosphofructo-1-kinase isozyme A antibody, phosphofructokinase 1 antibody, phosphofructokinase-M antibody, "6-phosphofructokinase, muscle type antibody", 6-phosphofructo-1-kinase antibody, "phosphofructokinase, polypeptide X antibody", PFK-A antibody, "phosphofructokinase, muscle antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PFK (muscle). The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Dog, Pig, Chicken, Bovine|
|Predict Reactivity Note||Rat (96%), Xenopus laevis (81%), Dog (99%), Pig (97%), Chicken (82%), Bovine (97%)|