|Applications||ELISA, ICC/IF, IHC-P, WB|
Not tested in other applications.
| ELISA||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||45 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , mouse brain , Rat liver , *Yeast wild type 303|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||phosphoglycerate kinase 1|
|Product Description||Rabbit Polyclonal antibody to PGK1 (phosphoglycerate kinase 1)|
|Background||The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]|
|Synonyms||MGC117307 antibody, MGC142128 antibody, MGC8947 antibody, MIG10 antibody, PGKA antibody, PGK1 antibody, phosphoglycerate kinase 1 antibody, cell migration-inducing gene 10 protein antibody, PRP 2 antibody, primer recognition protein 2 antibody|
|Cellular Localization||Cytoplasm |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat, Yeast|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (87%), Xenopus laevis (84%), Pig (96%), Chicken (88%), Sheep (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (96%)|