|Applications||ICC/IF, IHC-Fr, IHC-P, WB|
Not tested in other applications.
|IHC (Frozen sections)||1:100-1:1000*
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||25 kDa (note)
|Positive Controls||293T , A549 , H1299 , Neuro2A , GL261 , rat brain , PC-12 , mouse brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)|
|Product Description||Rabbit Polyclonal antibody to PGP9.5 (ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase))|
|Background||UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]|
|Synonyms||PARK5 antibody, PGP 9.5 antibody, PGP9.5 antibody, PGP95 antibody, Uch-L1 antibody, UCHL1 antibody, ubiquitin thioesterase L1 antibody, neuron cytoplasmic protein 9.5 antibody, ubiquitin C-terminal hydrolase antibody, ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) antibody|
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Xenopus Tropicalis, Pig, Bovine|
|Predict Reactivity Note||Xenopus laevis (80%), Xenopus Tropicalis (81%), Pig (88%), Bovine (87%)|