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1 .GTX83890 PIM2 antibody [8G10]

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PIM2 antibody [8G10]


  • Host / ClonalityMouse Monoclonal
  • Clone Name8G10
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX83890
Package Price Qty
100 μlUSD 339.00
Add to cart
 
  • Anti-PIM2 antibody [8G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in Western Blot (WB). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
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  • Datasheet
  • Application Information: PIM2 antibody [8G10]

Catalog Number GTX83890
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Product Name PIM2 antibody [8G10]
See all PIM2 products
ApplicationsICC/IF, IHC-P, WB
Application NoteRecommended Starting Dilutions:

WB 1:500
IHC 1:50
Flow 1:100
Optimal working dilutions should be determined experimentally by the end user.

Form SuppliedLiquid
PurificationProtein A/G purified

  • Specifications: PIM2 antibody [8G10]

Full Namepim-2 oncogene
BackgroundPromotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade | this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
SpecificityGTX83890 is specific for human PIM2 (NP_006866).
HostMouse
ClonalityMonoclonal
Clone Name8G10
IsotypeIgG2b
ImmunogenFull length human recombinant protein of human PIM2 (NP_006866) produced in HEK293T cell.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: PIM2 antibody [8G10]

Storage BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Storage InstructionFor short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Apoptosis > Anti-apoptosis
  • Cell Biology > Apoptosis > Anti-apoptosis
  • Cell Biology > Autophagy
More Hide
PIM2 antibody [8G10] validated data
  • Anti-PIM2 antibody [8G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83890
    • GTX83890 ICC/IF Image
      Anti-PIM2 mouse monoclonal antibody (GTX83890) immunofluorescent staining of COS7 cells transiently transfected with PIM2
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83890
    • GTX83890 ICC/IF Image
      Immunofluorescent staining of HepG2 cells using anti-PIM2 mouse monoclonal antibody (GTX83890).
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded prostate tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in Western Blot (WB). GTX83890
    • GTX83890 WB Image
      HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded liver tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83890
    • GTX83890 ICC/IF Image
      Anti-PIM2 mouse monoclonal antibody (GTX83890) immunofluorescent staining of COS7 cells transiently transfected with PIM2
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83890
    • GTX83890 ICC/IF Image
      Immunofluorescent staining of HepG2 cells using anti-PIM2 mouse monoclonal antibody (GTX83890).
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded prostate tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in Western Blot (WB). GTX83890
    • GTX83890 WB Image
      HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2.
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
  • Anti-PIM2 antibody [8G10] used in IHC (Formalin-fixed paraffin-embedded sections). GTX83890
    • GTX83890 IHC-P Image
      Immunohistochemical staining of paraffin-embedded liver tissue using anti-RC201933 mouse monoclonal antibody. (GTX83890, Dilution 1:50)
      Top
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