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  • PKA R2 (phospho Ser99) antibody [E151]

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1 .GTX61061 PKA R2 (phospho Ser99) antibody [E151]

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PKA R2 (phospho Ser99) antibody [E151]


  • Host / ClonalityRabbit Monoclonal
  • Clone NameE151
  • ApplicationsICC/IF, IHC-P, IP, WB
  • Species reactivityHuman, Mouse, Pig, Rat
Cat No: GTX61061
Package Price Qty
100 μlUSD 329.00
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  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in Western Blot (WB). GTX61061
  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX61061
  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in IHC (Formalin-fixed paraffin-embedded sections). GTX61061
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  • Application Information: PKA R2 (phospho Ser99) antibody [E151]

Catalog Number GTX61061
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Product Name PKA R2 (phospho Ser99) antibody [E151]
See all PKA R2 products
ApplicationsICC/IF, IHC-P, IP, WB
Application NoteWB: 1:2500, IHC-P: 1:250, IP: 1:20, ICC/IF: 1:500. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Predicted Target Size45 kDa (note)
Observed Size51 kDa kDa.
Positive ControlsK562 cell lysate, Hela cell lysate and lympho node metastatic tissue
Form SuppliedLiquid
ConcentrationBatch dependent (Please refer to the vial label for the specific concentration)
PurificationProtein A purified

  • Specifications: PKA R2 (phospho Ser99) antibody [E151]

Full Nameprotein kinase, cAMP-dependent, regulatory, type II, alpha
BackgroundcAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq, Jul 2008]
SynonymsPKR2 Antibody , PRKAR2A Antibody , PRKAR2 Antibody , protein kinase, cAMP-dependent, regulatory, type II, alpha Antibody
SpecificityThe antibody only detects PKA R2 phosphorylated on Serine 99. Phosphoserine 99 in Human corresponds to phosphoserine 96 in mouse.
HostRabbit
ClonalityMonoclonal
Clone NameE151
IsotypeIgG
ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Ser99 of human PKA R2
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Pig, Rat
ConjugationUnconjugated

  • Storage Conditions: PKA R2 (phospho Ser99) antibody [E151]

Storage BufferPBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide.
Storage InstructionShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,488.
PKA R2 (phospho Ser99) antibody [E151] validated data
  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in Western Blot (WB). GTX61061
    • GTX61061 WB Image
      WB analysis of (1) untreated and (2) Alkaline Phosphatase-treated K562 lysates using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:2500.
      Top
  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX61061
    • GTX61061 ICC/IF Image
      ICC/IF analysis of (A) untreated and (B) Alkaline Phosphatase treated HeLa cells using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:500.
      Top
  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in IHC (Formalin-fixed paraffin-embedded sections). GTX61061
    • GTX61061 IHC-P Image
      IHC-P analysis of (A) untreated and (B) Phosphatase treated lymph node metastatic tissues using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:250.
      Top
  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in Western Blot (WB). GTX61061
    • GTX61061 WB Image
      WB analysis of (1) untreated and (2) Alkaline Phosphatase-treated K562 lysates using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:2500.
      Top
  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX61061
    • GTX61061 ICC/IF Image
      ICC/IF analysis of (A) untreated and (B) Alkaline Phosphatase treated HeLa cells using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:500.
      Top
  • Anti-PKA R2 (phospho Ser99) antibody [E151] used in IHC (Formalin-fixed paraffin-embedded sections). GTX61061
    • GTX61061 IHC-P Image
      IHC-P analysis of (A) untreated and (B) Phosphatase treated lymph node metastatic tissues using PKA R2 (phospho Ser99) antibody [E151] at a dilution of 1:250.
      Top
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