GeneTex
  • Country / Location Selection

United States (US)

PKC beta 1 (phospho Thr642) antibody

Anti-PKC beta 1 (phospho Thr642) antibody used in Western Blot (WB). GTX25782

Cat. No. GTX25782

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human, Rat
Package
50 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Product Note

This antibody does not react with PKC beta 2 [pT641], alpha [pT638], nu [pT655], epsilon [pT71], iota [pT555], eta [pT655], or zeta [pT56] as determined by peptide competition experiments.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCbI that contains threonine 642. The sequence is conserved in rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380683

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase C beta , PKC-beta , PKCB , PKCI(2) , PKCbeta , PRKCB1 , PRKCB2

Cellular Localization

Cytoplasm,Nucleus,Membrane

Background

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PKC beta 1 (phospho Thr642) antibody used in Western Blot (WB). GTX25782

GTX25782 WB Image

WB (peptide competition) analysis of K562 cells stimulated with PMA using GTX25782 PKC beta 1 (phospho Thr642) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

REVIEW

There are currently no reviews for PKC beta 1 (phospho Thr642) antibody (GTX25782). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399