APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody does not react with PKC beta 2 [pT641], alpha [pT638], nu [pT655], epsilon [pT71], iota [pT555], eta [pT655], or zeta [pT56] as determined by peptide competition experiments.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA, 50% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCbI that contains threonine 642. The sequence is conserved in rat.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
RRID
AB_380683
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein kinase C beta , PKC-beta , PKCB , PKCI(2) , PKCbeta , PRKCB1 , PRKCB2
Cellular Localization
Cytoplasm,Nucleus,Membrane
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX25782 WB Image
WB (peptide competition) analysis of K562 cells stimulated with PMA using GTX25782 PKC beta 1 (phospho Thr642) antibody prior incubated with the non-phosphopeptide corresponding to the immunogen (Lane 2), a generic phosphothreonine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
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REFERENCE
REVIEW
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