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  • PKC theta (phospho Ser695) antibody

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1 .GTX109689 PKC theta (phospho Ser695) antibody

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PKC theta (phospho Ser695) antibody

Biological & Orthogonal

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsWB
  • Species reactivityHuman
Cat No: GTX109689
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-PKC theta (phospho Ser695) antibody used in Western Blot (WB). GTX109689
  • Anti-PKC theta (phospho Ser695) antibody used in Western Blot (WB). GTX109689
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  • Datasheet
  • Bio-info
  • Protocol & MSDS

  • Application Information: PKC theta (phospho Ser695) antibody

Catalog Number GTX109689
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Product Name PKC theta (phospho Ser695) antibody
See all PKC theta products
ApplicationsWB
Application Note
Suggested dilutionReference
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size82 kDa (note) IsoForm (6)
Positive ControlsHeLa , HepG2 , Molt-4 , Raji , MCF7 (500nM PMA treatment for 1hr)
Form SuppliedLiquid
Concentration0.66 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: PKC theta (phospho Ser695) antibody

Full Nameprotein kinase C, theta
BackgroundProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq]
SynonymsMGC126514 antibody, MGC141919 antibody, PRKCT antibody, nPKC-theta antibody, PRKCQ antibody, protein kinase C theta type antibody, "protein kinase C, theta antibody", Phospho PKC theta (pS695), Phospho PKC theta (Ser695), Phospho PKC theta (pSer695), PKC theta (pS695), PKC theta (pSer695), PKC theta phospho S695, PKC theta phospho Ser695, PKC theta (phospho S695), PKC theta (phospho Ser695)
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenCarrier-protein conjugated synthetic peptide corresponding to residues around human PKC theta (phospho Ser695). The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
 		Rhesus Monkey, Bovine
Predict Reactivity NoteRhesus Monkey (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: PKC theta (phospho Ser695) antibody

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Hypoxia
  • Disease Related > Alzheimer's disease
  • Disease Related > Diabetes
  • Immunology > Autoimmunity > Autoimmune diseases > Diabetes
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PKC theta (phospho Ser695) antibody validated data
  • Anti-PKC theta (phospho Ser695) antibody used in Western Blot (WB). GTX109689
    • GTX109689 WB Image  Biological & Orthogonal
      PKC theta (phospho Ser695) antibody detects PKC theta (phospho Ser695) protein by western blot analysis. Un-treated (-) and treated (+, 500nM PMA treatment for 1 hr) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Ser695) antibody (GTX109689) at a dilution of 1:500.
      Top
  • Anti-PKC theta (phospho Ser695) antibody used in Western Blot (WB). GTX109689
    • GTX109689 WB Image
      Sample (30 ug of whole cell lysate)
      A: Hela
      B: Hep G2 (GTX27900)
      C: Molt-4 (GTX27912)
      7.5% SDS PAGE
      PKC theta-phospho-S695 antibody
      GTX109689 diluted at 1:1000
      Top
  • Anti-PKC theta (phospho Ser695) antibody used in Western Blot (WB). GTX109689
    • GTX109689 WB Image  Biological & Orthogonal
      PKC theta (phospho Ser695) antibody detects PKC theta (phospho Ser695) protein by western blot analysis. Un-treated (-) and treated (+, 500nM PMA treatment for 1 hr) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta (phospho Ser695) antibody (GTX109689) at a dilution of 1:500.
      Top
  • Anti-PKC theta (phospho Ser695) antibody used in Western Blot (WB). GTX109689
    • GTX109689 WB Image
      Sample (30 ug of whole cell lysate)
      A: Hela
      B: Hep G2 (GTX27900)
      C: Molt-4 (GTX27912)
      7.5% SDS PAGE
      PKC theta-phospho-S695 antibody
      GTX109689 diluted at 1:1000
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensPRKCQ5588Q04759600448NP_00126957377.98
Homo sapiensPRKCQ5588Q04759600448NP_00126957467.57
Homo sapiensPRKCQ5588Q04759600448NP_00131019481.88
Homo sapiensPRKCQ5588Q04759600448NP_00131019567.57
Homo sapiensPRKCQ5588Q04759600448NM_001242413NP_00122934274.3
Homo sapiensPRKCQ5588Q04759600448NM_006261NP_00624881.88
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-ProClin.pdf

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