PLK1 antibody [N2C2], Internal
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||68 kDa (note)
|Positive Controls||A431 , H1299 , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||polo-like kinase 1|
|Product Description||Rabbit Polyclonal antibody to PLK1 (polo-like kinase 1)|
|Background||Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. |
|Synonyms||PLK antibody, STPK13 antibody, PLK1 antibody, serine/threonine-protein kinase 13 antibody, polo (Drosophia)-like kinase antibody, polo like kinase antibody, cell cycle regulated protein kinase antibody, serine/threonine-protein kinase PLK1 antibody, PLK-1 antibody, polo-like kinase 1 antibody|
|Cellular Localization||Nucleus. This antibody also stains PLK1 on spindles, kinetochores, and midbodies.|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PLK1. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Xenopus laevis, Chicken, Bovine|
|Predict Reactivity Note||Mouse (95%), Rat (95%), Xenopus laevis (80%), Chicken (80%), Bovine (95%)|