POLH antibody [N1N3]
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||78 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||polymerase (DNA directed), eta|
|Product Description||Rabbit polyclonal antibody to POLH (polymerase (DNA directed), eta)|
|Background||This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. [provided by RefSeq]|
|Synonyms||FLJ16395 antibody, FLJ21978 antibody, RAD30 antibody, RAD30A antibody, XP-V antibody, XPV antibody, POLH antibody, xeroderma pigmentosum variant type protein antibody, DNA polymerase eta antibody, RAD30 homolog A antibody, "polymerase (DNA directed), eta antibody"|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human POLH. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Bovine|
|Predict Reactivity Note||Mouse (92%), Rat (89%), Bovine (83%)|