|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||46 kDa (note)
|Positive Controls||A431 , HepG2 , Neuro2A , GL261 , C8D30 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||proteasome (prosome, macropain) 26S subunit, ATPase, 5|
|Product Description||Rabbit Polyclonal antibody to PSMC5 (proteasome (prosome, macropain) 26S subunit, ATPase, 5)|
|Background||The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. [provided by RefSeq]|
|Synonyms||S8 antibody, SUG-1 antibody, SUG1 antibody, TBP10 antibody, TRIP1 antibody, p45 antibody, p45/SUG antibody, PSMC5 antibody, proteasome subunit p45 antibody, Tat-binding protein homolog 10 antibody, thyroid hormone receptor-interacting protein 1 antibody, 26S proteasome AAA-ATPase subunit RPT6 antibody, proteasome 26S subunit ATPase 5 antibody, proteasome 26S ATPase subunit 5 antibody, 26S protease regulatory subunit 8 antibody, MSUG1 protein antibody, thyroid receptor interactor 1 antibody, proteasome (prosome, macropain) 26S subunit, ATPase, 5 antibody|
|Cellular Localization||Cytoplasm (Potential) , Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PSMC5. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)|