|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||30 kDa (note)
|Positive Controls||A549 , H1299 , HCT116 , MCF-7 , NIH-3T3 , JC , BCL-1 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)|
|Product Description||Rabbit Polyclonal antibody to PSME3 (proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki))|
|Background||The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq]|
|Synonyms||Ki antibody, PA28-gamma antibody, PA28G antibody, REG-GAMMA antibody, PSME3 antibody, Ki nuclear autoantigen antibody, proteasome activator 28 subunit gamma antibody, PA28gamma antibody, proteasome activator 28-gamma antibody, 11S regulator complex subunit gamma antibody, 11S regulator complex gamma subunit antibody, activator of multicatalytic protease subunit 3 antibody, Ki antigen antibody, proteasome activator complex subunit 3 antibody, "proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) antibody"|
|Cellular Localization||Nucleus , Cytoplasm (By similarity) |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PSME3. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Pig, Chicken, Chimpanzee|
|Predict Reactivity Note||Xenopus laevis (88%), Pig (100%), Chicken (94%), Chimpanzee (99%)|