|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||47 kDa (note)
|Positive Controls||293T, A549 , H1299 , HCT116 , MCF-7 , BCL-1 , NIH-3T3 , PC-12 , *Human embryonic stem cell|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||phosphatase and tensin homolog|
|Product Description||Rabbit Polyclonal antibody to PTEN (phosphatase and tensin homolog)|
|Background||This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq]|
|Synonyms||10q23del antibody, BZS antibody, DEC antibody, GLM2 antibody, MGC11227 antibody, MHAM antibody, MMAC1 antibody, PTEN1 antibody, TEP1 antibody, PTEN antibody, mutated in multiple advanced cancers 1 antibody, phosphatase and tensin-like protein antibody, phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody, MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody, phosphatase and tensin homolog antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PTEN. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Dog (100%), Pig (99%), Rhesus Monkey (100%), Bovine (99%)|