PTP1B antibody [N1N3]
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||50 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||protein tyrosine phosphatase, non-receptor type 1|
|Product Description||Rabbit polyclonal antibody to PTP1B (protein tyrosine phosphatase, non-receptor type 1)|
|Background||The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. [provided by RefSeq]|
|Synonyms||PTP1B antibody, PTPN1 antibody, tyrosine-protein phosphatase non-receptor type 1 antibody, protein-tyrosine phosphatase 1B antibody, protein tyrosine phosphatase 1B antibody, "protein tyrosine phosphatase, placental antibody", "protein tyrosine phosphatase, non-receptor type 1 antibody"|
|Cellular Localization||Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PTP1B. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Zebrafish, Pig, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Mouse (94%), Rat (95%), Zebrafish (86%), Pig (99%), Chicken (96%), Rhesus Monkey (100%), Bovine (96%)|