PUF60 antibody [GT3510]
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||mouse brain , rat brain , 293T , A431 , HeLa , HepG2|
(Please refer to the vial label for the specific concentration)|
|Purification||Affinity purified by Protein G.|
|Full Name||poly-U binding splicing factor 60KDa|
|Product Description||Mouse Monoclonal antibody to PUF60|
|Background||The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene. [provided by RefSeq]|
|Synonyms||SIAHBP1 Antibody, FIR Antibody, poly-U binding splicing factor 60KDa Antibody, RoBPI Antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human PUF60. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Chicken (94%), Bovine (96%)|