Paxillin (phospho Tyr118) antibody [GT712]

Host / ClonalityMouse Monoclonal
Clone NameGT712
ApplicationsICC/IF, WB
Species reactivityHuman, Rat
★★★★★ ( 1 )

Cat No:

GTX633633

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Bio-info
Protocol & MSDS

Application Information: Paxillin (phospho Tyr118) antibody [GT712]

Catalog Number

GTX633633

Product Name

Paxillin (phospho Tyr118) antibody [GT712]

See all Paxillin products

Applications

ICC/IF, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	Assay-dependent dilution
Western Blot (WB)	1:500-1:10000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

65 kDa (note) IsoForm

(3)

Positive Controls

Rat2 , A431 (1 mM Pervanadate treatment for 0.5 hr)

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Affinity purified by Protein G.

Specifications: Paxillin (phospho Tyr118) antibody [GT712]

Full Name	paxillin
Synonyms	PXN antibody , Paxillin (phospho Tyr118) antibody [GT712] antibody , Paxillin (phospho Tyr118) antibody , Paxillin antibody
Cellular Localization	Cytoplasm , cytoskeleton , Cell junction , focal adhesion
Host	Mouse
Clonality	Monoclonal
Clone Name	GT712
Isotype	IgG1
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Paxillin (phospho Tyr118). The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Rat
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Mouse, Chicken
Predict Reactivity Note	Mouse (100%), Chicken (100%)
Conjugation	Unconjugated

Storage Conditions: Paxillin (phospho Tyr118) antibody [GT712]

Storage Buffer	PBS
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Type of cancer > Lung > Non-small cell Cell Biology > Cytoskeleton Signal Transduction > ECM/Cytoskeleton > Cell Adhesion Signal Transduction > ECM/Cytoskeleton > Cytoskeleton More Hide

Paxillin (phospho Tyr118) antibody [GT712] validated data

GTX633633 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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GTX633633 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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GTX633633 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX633633 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Top

StarReview
Paxillin (phospho Tyr118) antibody [GT712]
Rating : ★★★★★ ( Average 5.0 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence (ICC/IF)(1) :★★★★★

Anonymous StarReview submitted on 21 Sep 2016
Score : ★★★★★
Application Tested : ICC/IF
Sample Species : Human
Sample : ARPE-19 cells
Blocking conditions
Blocking Buffer : 3% BSA and 0.02% Triton-X100 in PBS
Incubation Time & Temperature : RT (℃)/1hr
Primary antibody
Dilution : 1:50( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	PXN	5829	P49023, Q5HYA4	602505		NP_001230685	66.22
Homo sapiens	PXN	5829	P49023, Q5HYA4	602505	NM_001080855	NP_001074324	64.52
Homo sapiens	PXN	5829	P49023, Q5HYA4	602505	NM_002859	NP_002850	60.98

Cross-react Bioinfo
Species	Gene name	Gene ID	SwissProt	Isoform (mRNA)	Protein Accession#	MW
Mus musculus	Pxn	19303	Q3UG90	NM_133915	NP_598676	64.52
Mus musculus	Pxn	19303	Q3UG90	NM_011223	NP_035353	60.82
Rattus norvegicus	Pxn	360820	Q66H76	NM_001012147	NP_001012147	64.03

*MSDS

MSDS-PBS.pdf

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