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  • Paxillin (phospho Tyr118) antibody [GT712]

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1 .GTX633633 Paxillin (phospho Tyr118) antibody [GT712]

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Paxillin (phospho Tyr118) antibody [GT712]

Biological & Orthogonal

  • Host / ClonalityMouse Monoclonal
  • Clone NameGT712
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Rat
  • ★★★★★   (  1  )
Cat No: GTX633633
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
  • Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
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  • Datasheet
  • Star Review (1)
    Reference (0)
  • Bio-info
  • Protocol & MSDS
  • Application Information: Paxillin (phospho Tyr118) antibody [GT712]

Catalog Number GTX633633
★★★★★
( 1 )
Publication ( 0 )
Product Name Paxillin (phospho Tyr118) antibody [GT712]
See all Paxillin products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)Assay-dependent dilution  
Western Blot (WB)1:500-1:10000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size65 kDa (note) IsoForm (3)
Positive ControlsRat2 , A431 (1 mM Pervanadate treatment for 0.5 hr)
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationAffinity purified by Protein G.

  • Specifications: Paxillin (phospho Tyr118) antibody [GT712]

Full Namepaxillin
SynonymsPXN antibody , Paxillin (phospho Tyr118) antibody [GT712] antibody , Paxillin (phospho Tyr118) antibody , Paxillin antibody
Cellular LocalizationCytoplasm , cytoskeleton , Cell junction , focal adhesion
HostMouse
ClonalityMonoclonal
Clone NameGT712
IsotypeIgG1
ImmunogenCarrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Paxillin (phospho Tyr118). The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Chicken
Predict Reactivity NoteMouse (100%), Chicken (100%)
ConjugationUnconjugated

  • Storage Conditions: Paxillin (phospho Tyr118) antibody [GT712]

Storage BufferPBS
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Lung > Non-small cell
  • Cell Biology > Cytoskeleton
  • Signal Transduction > ECM/Cytoskeleton > Cell Adhesion
  • Signal Transduction > ECM/Cytoskeleton > Cytoskeleton
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Paxillin (phospho Tyr118) antibody [GT712] validated data
  • Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
    • GTX633633 WB Image
      Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
    • GTX633633 WB Image
      Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
    • GTX633633 WB Image
      Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:8000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
      Top
  • Anti-Paxillin (phospho Tyr118) antibody [GT712] used in Western Blot (WB). GTX633633
    • GTX633633 WB Image
      Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Tyr118) antibody [GT712] (GTX633633) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
      Top
  • StarReview
  • Paxillin (phospho Tyr118) antibody [GT712]

  • Rating : ★★★★★ ( Average 5.0 based on 1 users reviews)
  • Immunocytochemistry/ Immunofluorescence (ICC/IF)(1) :★★★★★
  • Anonymous StarReview submitted on 21 Sep 2016
  • Score : ★★★★★
  • Application Tested : ICC/IF
  • Sample Species : Human
  • Sample : ARPE-19 cells
  • Blocking conditions
  • Blocking Buffer : 3% BSA and 0.02% Triton-X100 in PBS
  • Incubation Time & Temperature : RT (℃)/1hr
  • Primary antibody
  • Dilution : 1:50( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensPXN5829P49023, Q5HYA4602505NP_00123068566.22
Homo sapiensPXN5829P49023, Q5HYA4602505NM_001080855NP_00107432464.52
Homo sapiensPXN5829P49023, Q5HYA4602505NM_002859NP_00285060.98

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusPxn19303Q3UG90NM_133915NP_59867664.52
Mus musculusPxn19303Q3UG90NM_011223NP_03535360.82
Rattus norvegicusPxn360820Q66H76NM_001012147NP_00101214764.03
    *MSDS
    MSDS-PBS.pdf
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