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Peanut Lectin (PE)

Cat. No. GTX01509

Reference 4

Application

 ICC/IF, FACS

Species

 Arachis hypogaea
Package
500 μg ($169)
See all Peanut Lectin products
PRODUCT

Summary

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:50-1:200
FACS 1:100-1:500
Not tested in other applications.

Product Note

Carbohydrate-Binding Specificity of Peanut Lectin : Galβ1, 3 GalNAc>>GalNAc>Gal

PROPERTIES

Form

Liquid

Buffer

10mM Bicarbonate (pH8.2), 150mM NaCl, 0.1mM Calcium Chloride

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Native Protein

Expression System

Native Protein

Purification


Purified from Peanut

Conjugation

Phycoerythrin (PE)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Peanut Lectin , PNA , PNA lectin , Arachis hypogaea , Arachis hypogaea lectin , Peanut agglutinin lectin , Peanut agglutinin

Background

Peanut lectin (PNA) is an identical tetrameric carbohydrate free protein with MW of 110 kDa. Thomsen-Friedenreich antigen, T-antigen (Galβ1, 3GalNAc) is present on blood group M &N glycoproteins (after removal of sialic acid with neuraminidase), glyconjugates (Mucin type), gangliosides and many glycolipids. T antigen is rarely expressed on normal coloncytes whereas cells of malignant, premalignant cells express this antigen. Peanut lectin has widely been used to detect T antigen in malignant and premalignant cells. Peanut lectin contains one atom of Ca++ and Mg++ per unit.

Inhibiting/Eluting sugars: 200 mM Galactose
Carbohydrate-Binding Specificity of PNA: Galβ1, 3 GalNAc>>>>>GalNAc>Gal

Research Area

REFERENCE

Application Reference

Ohaneje UL et al. Animals (Basel) 2021; 11 (12) The Re-Addition of Seminal Plasma after Thawing Does Not Improve Buck Sperm Quality Parameters.
C?mara DR et al. Reprod Domest Anim 2021; (Epub) Mitoquinone does not improve sperm cryo-resistance in bulls.
Yamamoto T et al. Sci Transl Med 2015; 7 (298) : 298ra120 Quality and quantity of TFH cells are critical for broad antibody development in SHIVAD8 infection.
Petrovas C et al. J Clin Invest 2012; 122 (9) : 3281-94 CD4 T follicular helper cell dynamics during SIV infection.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 169