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1 .GTX117411 Periplakin antibody [N1N2], N-term

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Periplakin antibody [N1N2], N-term


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, WB
  • Species reactivityHuman, Mouse
Cat No: GTX117411
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Periplakin antibody [N1N2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX117411
  • Anti-Periplakin antibody [N1N2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX117411
  • Anti-Periplakin antibody [N1N2], N-term used in Western Blot (WB). GTX117411
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  • Application Information: Periplakin antibody [N1N2], N-term

Catalog Number GTX117411
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Product Name Periplakin antibody [N1N2], N-term
See all Periplakin products
ApplicationsIHC-P, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size205 kDa (note) IsoForm (2)
Positive ControlsU87-MG
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Periplakin antibody [N1N2], N-term

Full Nameperiplakin
BackgroundThe protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq]
SynonymsKIAA0568 antibody, MGC134872 antibody, PPL antibody, 190 kDa paraneoplastic pemphigus antigen antibody, periplakin antibody, 195 kDa cornified envelope precursor protein antibody
Cellular LocalizationCell junction , desmosome , Cytoplasm , cytoskeleton , Cell membrane (By similarity) , Nucleus (By similarity) , Mitochondrion (By similarity)
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the N-terminus region of human Periplakin. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat
Predict Reactivity NoteRat (91%)
ConjugationUnconjugated

  • Storage Conditions: Periplakin antibody [N1N2], N-term

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cell Biology > Cytoskeleton
  • Signal Transduction > ECM/Cytoskeleton > Cytoskeleton
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Periplakin antibody [N1N2], N-term validated data
  • Anti-Periplakin antibody [N1N2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX117411
    • GTX117411 IHC-P Image
      Periplakin antibody [N1N2], N-term detects Periplakin protein at membrane on mouse prostate by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse prostate.
      Periplakin antibody [N1N2], N-term (GTX117411) dilution: 1:500.
      Top
  • Anti-Periplakin antibody [N1N2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX117411
    • GTX117411 IHC-P Image
      Periplakin antibody [N1N2], N-term detects PPL protein at cytosol on U87 xenograft by immunohistochemical analysis.
      Sample: Paraffin-embedded U87 xenograft.
      Periplakin antibody [N1N2], N-term (GTX117411) dilution: 1:500.
      Top
  • Anti-Periplakin antibody [N1N2], N-term used in Western Blot (WB). GTX117411
    • GTX117411 WB Image
      Sample (30 ug of whole cell lysate)
      A: U87-MG
      5% SDS PAGE
      GTX117411 diluted at 1:5000
      Top
  • Anti-Periplakin antibody [N1N2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX117411
    • GTX117411 IHC-P Image
      Periplakin antibody [N1N2], N-term detects Periplakin protein at membrane on mouse prostate by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse prostate.
      Periplakin antibody [N1N2], N-term (GTX117411) dilution: 1:500.
      Top
  • Anti-Periplakin antibody [N1N2], N-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX117411
    • GTX117411 IHC-P Image
      Periplakin antibody [N1N2], N-term detects PPL protein at cytosol on U87 xenograft by immunohistochemical analysis.
      Sample: Paraffin-embedded U87 xenograft.
      Periplakin antibody [N1N2], N-term (GTX117411) dilution: 1:500.
      Top
  • Anti-Periplakin antibody [N1N2], N-term used in Western Blot (WB). GTX117411
    • GTX117411 WB Image
      Sample (30 ug of whole cell lysate)
      A: U87-MG
      5% SDS PAGE
      GTX117411 diluted at 1:5000
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensPPL5493O60437602871BC114620AAI14621204.7
Homo sapiensPPL5493O60437602871NM_002705NP_002696204.73

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusPpl19041Q3UQD7NM_008909NP_032935203.84
Rattus norvegicusPpl302934NM_001106976NP_001100446204.2
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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All products are for research use only—Not for use in diagnostic procedures.