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Catalog Number
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GTX29337
(
0
)
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Product Name
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Phosphothreonine antibody
|
| Applications | ELISA, IP, WB |
| Application Note | ELISA(kinase assay): Use at 0.5 μg/mLWestern blot: Use at 4μg/mLIP: Use at 10 μg/250 μg protein sampleWill detect 100 ng of phosvitin in Western Blots and 0.5 ng of phosvitin with ELISA.Can be used for non-radioactive protein kinase assay (ELISA) using biotinylated peptide substrate and immunoblotting of abundant phosphoproteins. It is not recommended for immunoblotting of trace cellular phosphoproteins. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation. |
| Positive Controls | Use mouse brain extract for immunoblotting. Use synthetic phosphopeptide (phosphorylated on threonine) for ELISA. |
| Form Supplied | Liquid |
| Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Immunogen affinity purified |
| Purification Note | Immunoaffinity chromatography with phosphothreonine-agarose. |