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1 .GTX29337 Phosphothreonine antibody

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Phosphothreonine antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsELISA, IP, WB
Cat No: GTX29337
Package Price Qty
100 μgUSD 359.00
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  • Anti-Phosphothreonine antibody used in Western Blot (WB). GTX29337
  • Anti-Phosphothreonine antibody used in ELISA. GTX29337
  • Anti-Phosphothreonine antibody used in Western Blot (WB). GTX29337
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  • Datasheet
  • Application Information: Phosphothreonine antibody

Catalog Number GTX29337
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Product Name Phosphothreonine antibody
See all Phosphothreonine products
ApplicationsELISA, IP, WB
Application NoteELISA(kinase assay): Use at 0.5 μg/mLWestern blot: Use at 4μg/mLIP: Use at 10 μg/250 μg protein sampleWill detect 100 ng of phosvitin in Western Blots and 0.5 ng of phosvitin with ELISA.Can be used for non-radioactive protein kinase assay (ELISA) using biotinylated peptide substrate and immunoblotting of abundant phosphoproteins. It is not recommended for immunoblotting of trace cellular phosphoproteins. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.
Positive ControlsUse mouse brain extract for immunoblotting. Use synthetic phosphopeptide (phosphorylated on threonine) for ELISA.
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationImmunogen affinity purified
Purification NoteImmunoaffinity chromatography with phosphothreonine-agarose.

  • Specifications: Phosphothreonine antibody

BackgroundPhosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
SpecificityReacts with free phosphothreonine but does not react with phosphoserine, threonine or phosphotyrosine.
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenChemical / Small Molecule conjugated to KLH.
ConjugationUnconjugated

  • Storage Conditions: Phosphothreonine antibody

Storage BufferTBS with 0.01% sodium azide
Storage InstructionKeep as concentrated solution, aliquot and store at 4°C. Do not freeze.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Phosphothreonine antibody validated data
  • Anti-Phosphothreonine antibody used in Western Blot (WB). GTX29337
    • GTX29337 WB Image
      Western blot analysis of mouse spleen extract probed with GTX29337.
      Left Lane: Vanadium treated
      Right lane: Untreated
      Top
  • Anti-Phosphothreonine antibody used in ELISA. GTX29337
    • GTX29337 ELISA Image
      Antibody Capture ELISA. Label: immobilized antigen
      Top
  • Anti-Phosphothreonine antibody used in Western Blot (WB). GTX29337
    • GTX29337 WB Image
      Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
      Top
  • Anti-Phosphothreonine antibody used in Western Blot (WB). GTX29337
    • GTX29337 WB Image
      Western blot analysis of mouse spleen extract probed with GTX29337.
      Left Lane: Vanadium treated
      Right lane: Untreated
      Top
  • Anti-Phosphothreonine antibody used in ELISA. GTX29337
    • GTX29337 ELISA Image
      Antibody Capture ELISA. Label: immobilized antigen
      Top
  • Anti-Phosphothreonine antibody used in Western Blot (WB). GTX29337
    • GTX29337 WB Image
      Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)
      Top
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