GeneTex

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PolymerPlus HRP High Sensitivity anti-mouse/rat/rabbit Detection System (Ready-to-Use)

Cat. No. GTX83397

Application

 WB, IHC

Reactivity

 Mouse, Rat, Rabbit
Package
1 kit ($249)
See all Polymer HRP anti-Mouse/rat/Rabbit products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Target Isotype

IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

antimouse HRP polymer , anti-rat HRP polymer , anti-rabbit HRP polymer , Ultra High Sensitivity IHC polymer , anti-mouse HRP polymer , antirabbit HRP polymer , antirat HRP polymer , anti rat HRP polymer , anti mouse HRP polymer , anti rabbit HRP polymer

Background

Immunohistochemistry (IHC) is the localization of antigens in tissue sections/cells using antibodies as specific reagents. Visualization of antibody signal is often significantly improved using the biotin-avidin amplification system. This system, however, adds significant time to experimental protocols and results in higher background due to the presence of endogenous biotin in tissue.

GeneTex’s PolymerPlus anti-mouse/rat/rabbit IgG (H+L) uses the power of next-generation Polymer technology to provide dramatic signal amplification without the need for biotin. This two-step kit has been specially designed to provide enhanced detection of low abundance antigens. It has a sensitivity superior to that of the biotin-avidin system. It has been shown to provide increased sensitivity and detection, while reducing the incubation time and required quantity of primary antibody. This reagent can be used to enhance staining of membranes, cytoplasmic and nuclear antigens. It provides the user with a rapid and easy to use IHC detection system.

Features and Benefits
‧ Highest possible sensitivity for IHC detection
‧ Specially designed for low abundant antigens
‧ Lower background: Amplification without biotin avoids background from endogenous biotin
‧ Faster: Staining time reduced to 20 minutes!
‧ Compatible with either manual or automated systems
‧ Better results on difficult-to-work antibodies and nuclear staining
‧ Save cost due to further dilution of primary antibody

REFERENCE

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REVIEW

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Package List Price ($)
$ 249