APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human Androgen Receptor.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
AIS,AR,AR8,DHTR,HUMARA,HYSP1,KD,NR3C4,SBMA,SMAX1,TFM,androgen receptor,Androgen Receptor
Cellular Localization
Nucleus,Cytoplasm
Background
The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]
Database
Research Area
DATA IMAGES
![Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536](/templates/default/js/lightbox/images/loading.gif "Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536")
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GTX01536 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor antibody [GT1228] (GTX01536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 11306464
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GTX01536 WB Image
WB analysis of mouse testis tissue lysate using GTX01536 Androgen Receptor antibody [GT1228].
Dilution : 1:1000
Loading : 25 μg
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GTX01536 IHC-P Image
IHC-P analysis of human breast tissue section using GTX01536 Androgen Receptor antibody [GT1228].
Dilution : 1:100
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GTX01536 ICC/IF Image
ICC/IF analysis of LNCaP cells using GTX01536 Androgen Receptor antibody [GT1228].
Blue : DAPI
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REFERENCE
There are currently no references for Androgen Receptor antibody [GT1228] (GTX01536). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Androgen Receptor antibody [GT1228] (GTX01536). Be the first to share your experience with this product.
![Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536](/upload/website/prouct_img/small/GTX01536/GTX01536_4000000090_20200410_WB_w_23053121_428.webp "Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536")
![Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536](/upload/website/prouct_img/small/GTX01536/GTX01536_20200508_WB_w_23053121_665.webp "Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536")
![Anti-Androgen Receptor antibody [GT1228] used in IHC (Paraffin sections) (IHC-P). GTX01536](/upload/website/prouct_img/small/GTX01536/GTX01536_20200508_IHC-P_w_23053121_724.webp "Anti-Androgen Receptor antibody [GT1228] used in IHC (Paraffin sections) (IHC-P). GTX01536")
![Anti-Androgen Receptor antibody [GT1228] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01536](/upload/website/prouct_img/small/GTX01536/GTX01536_20200508_ICCIF_w_23053121_194.webp "Anti-Androgen Receptor antibody [GT1228] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01536")
![Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536](/upload/website/prouct_img/normal/GTX01536/GTX01536_4000000090_20200410_WB_w_23053121_428.webp "Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536")
![Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536](/upload/website/prouct_img/normal/GTX01536/GTX01536_20200508_WB_w_23053121_665.webp "Anti-Androgen Receptor antibody [GT1228] used in Western Blot (WB). GTX01536")
![Anti-Androgen Receptor antibody [GT1228] used in IHC (Paraffin sections) (IHC-P). GTX01536](/upload/website/prouct_img/normal/GTX01536/GTX01536_20200508_IHC-P_w_23053121_724.webp "Anti-Androgen Receptor antibody [GT1228] used in IHC (Paraffin sections) (IHC-P). GTX01536")
![Anti-Androgen Receptor antibody [GT1228] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01536](/upload/website/prouct_img/normal/GTX01536/GTX01536_20200508_ICCIF_w_23053121_194.webp "Anti-Androgen Receptor antibody [GT1228] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01536")