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Beclin 1 antibody

Anti-Beclin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31721
Anti-Beclin 1 antibody used in Western Blot (WB). GTX31721
Anti-Beclin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31721

Cat. No. GTX31721

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all Beclin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ICC/IF 1 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

A-431 Cell Slide(GTX25525) , A431 Cell Lysate(GTX27909)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Beclin-1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beclin-1.The immunogen is located within the last 50 amino acids of Beclin-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

beclin 1 , ATG6 , VPS30 , beclin1

Cellular Localization

Cytoplasm,Endosome membrane,Endoplasmic reticulum membrane,Endosome,Nucleus

Background

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Beclin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31721

GTX31721 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31721 Beclin 1 antibody.
Working concentration : 1 μg/ml

Anti-Beclin 1 antibody used in Western Blot (WB). GTX31721

GTX31721 WB Image

WB analysis of A431 cell lysate using GTX31721 Beclin 1 antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

Anti-Beclin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31721

GTX31721 ICC/IF Image

ICC/IF analysis of A431 cells using GTX31721 Beclin 1 antibody.
Working concentration : 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399