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CD34 antibody [MSVA-034R] HistoMAX™

HistoMAX
Anti-CD34 antibody [MSVA-034R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04386
Anti-CD34 antibody [MSVA-034R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04386
Anti-CD34 antibody [MSVA-034R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04386

Cat. No. GTX04386

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 MSVA-034R

Isotype

 IgG

Application

 IHC-P

Reactivity

 Human
Package
500 μl ($629),
100 μl ($299)
See all CD34 products
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on the testing result, it stains almost identically for pH 6.0 to 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Liver: A moderate to strong, predominantly membranous staining of endothelial cells should be seen in portal vessels and periportal sinusoidal endothelial cells. Endothelial cells located more remotely from the portal field show a rapidly decreasing CD34 expression level.

Negative Control

Liver: Hepatocytes should always stain CD34 negative. Sinusoidal cells remote from the portal fields must also be CD34 negative.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CD34 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD34 molecule

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-CD34 antibody [MSVA-034R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04386

GTX04386 IHC-P Image

IHC-P analysis of human mature placenta tissue section using GTX04386 CD34 antibody [MSVA-034R] HistoMAX™.
Strong CD34 immunostaining of blood vesels in a mature placenta.

Anti-CD34 antibody [MSVA-034R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04386

GTX04386 IHC-P Image

IHC-P analysis of human gastrointestinal stromal tumor tissue section using GTX04386 CD34 antibody [MSVA-034R] HistoMAX™.
Gastrointestinal stromal tumor with strong CD34 expression.

Anti-CD34 antibody [MSVA-034R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04386

GTX04386 IHC-P Image

IHC-P analysis of human liver tissue section using GTX04386 CD34 antibody [MSVA-034R] HistoMAX™.
In the liver, CD34 is only seen in portal vessels and periportal sinusoidal.

REFERENCE

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REVIEW

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Package List Price ($)
$ 629
$ 299