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Chk2 antibody [8F12]

Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295
Anti-Chk2 antibody [8F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70295
Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295

Cat. No. GTX70295

Host

Mouse

Clonality

Monoclonal

Clone Name

8F12

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

HepG2, MCF-7, or LN-Cap total cell extract

Product Note

GTX70295 is specific for human CHK2 protein.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant CHK2 protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_369315

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1

Cellular Localization

nucleoplasm

Background

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295

GTX70295 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk2 antibody [8F12] (GTX70295) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Chk2 antibody [8F12] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70295

GTX70295 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using (GTX70295) antibody at 1:500 dilution.

Anti-Chk2 antibody [8F12] used in Western Blot (WB). GTX70295

GTX70295 WB Image

The data was published in the journal PLoS One in 2014. PMID: 24933654

REFERENCE

REVIEW

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Package List Price ($)
$ 399