Cytochrome C antibody [CYCS/3128R]

Cat. No. GTX02626

Host	Rabbit
Clonality	Monoclonal
Clone Name	CYCS/3128R
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Mouse, Rat, Drosophila, Dog, Frog, Horse, pigeon
Package	100 μg ($529)

See all Cytochrome C products

Datasheet

View product citations for antibody GTX02626 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2 μg/ml
ICC/IF	1-2 μg/ml
IHC-P	0.25-0.5 μg/ml
FACS	1-2 μg/million cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Product Note

This antibody recognizes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e. cytochrome in the mitochondria with heme attached).

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

200 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human CYCS protein. An epitope within amino acids 93-104 of pigeon Cytochrome C.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c, somatic , CYC , HCS , THC4

Background

This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]

Database

Gene ID: 54205 CYCS
UniProt: P99999 CYCS

Research Area

DATA IMAGES

GTX02626 IHC-P Image

IHC-P analysis of human liver tissue section using GTX02626 Cytochrome C antibody [CYCS/3128R].

GTX02626 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX02626 Cytochrome C antibody [CYCS/3128R].
Blue : Primary antibody
Red : Isotype control

GTX02626 ICC/IF Image

ICC/IF analysis of PFA fixed HeLa cells using GTX02626 Cytochrome C antibody [CYCS/3128R].
Green : Primary antibody

GTX02626 WB Image

WB analysis of human heart tissue lysate using GTX02626 Cytochrome C antibody [CYCS/3128R].

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 529

Qty