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Cytochrome C antibody [GT1711]

Anti-Cytochrome C antibody [GT1711] used in Western Blot (WB). GTX634187
Anti-Cytochrome C antibody [GT1711] used in IHC (Paraffin sections) (IHC-P). GTX634187
Anti-Cytochrome C antibody [GT1711] used in Western Blot (WB). GTX634187

Cat. No. GTX634187

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1711

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all Cytochrome C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human Cytochrome C recombinant protein.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888423

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c, somatic , CYC , HCS , THC4

Cellular Localization

Mitochondrion matrix

Background

This gene encodes cytochrome c, a component of the electron transport chain in mitochondria. The heme group of cytochrome c accepts electrons from the b-c1 complex and transfers electrons to the cytochrome oxidase complex. Cytochrome c is also involved in initiation of apoptosis. Upon release of cytochrome c to the cytoplasm, the protein binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Many cytochrome c pseudogenes exist, scattered throughout the human genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Cytochrome C antibody [GT1711] used in Western Blot (WB). GTX634187

GTX634187 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [GT1711] (GTX634187) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Cytochrome C antibody [GT1711] used in IHC (Paraffin sections) (IHC-P). GTX634187

GTX634187 IHC-P Image

Cytochrome C antibody [GT1711] detects Cytochrome C protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
Cytochrome C stained by Cytochrome C antibody [GT1711] (GTX634187) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Cytochrome C antibody [GT1711] used in Western Blot (WB). GTX634187

GTX634187 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome C antibody [GT1711] (GTX634187) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations