Estrogen Receptor beta (phospho Ser105) antibody

Cat. No. GTX04765

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human, Mouse
Package	100 μl ($399)

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Datasheet

View product citations for antibody GTX04765 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
ICC/IF	1:100-1:500
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Predict Reactivity

Rat, Sheep, Dog, Chicken, Horse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150 mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ESR2(Accession Q92731), corresponding to amino acid residues around phosphorylated Ser105.

Purification

Purified by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ERBETA,ESR2,ESRB,ESRBETA,ESTRB,Erb,NR3A2,estrogen receptor 2,ER beta,Estrogen Receptor beta

Cellular Localization

Nucleus

Background

This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2100 ESR2
UniProt: Q92731 ESR2

Research Area

DATA IMAGES

GTX04765 WB Image

WB analysis of various samples with heat-shocked treatment using GTX04765 Estrogen Receptor beta (phospho Ser105) antibody. The first lane was treated with blocking peptide.
Lane 1 and 2 : A2780 whole cell lysate
Lane 3 : Raw264.7 whole cell lysate

GTX04765 ICC/IF Image

ICC/IF analysis of PFA-fixed HUVEC cells using GTX04765 Estrogen Receptor beta (phospho Ser106) antibody.
Permeabilization : 0.1% saponin
Dilution : 1:400

GTX04765 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using serial diluted GTX04765 Estrogen Receptor beta (phospho Ser106) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1 μg/mL

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

Qty