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FOXO4 (phospho Ser197) antibody

Anti-FOXO4 (phospho Ser197) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50197
Anti-FOXO4 (phospho Ser197) antibody used in Western Blot (WB). GTX50197
Anti-FOXO4 (phospho Ser197) antibody used in IHC (Paraffin sections) (IHC-P). GTX50197

Cat. No. GTX50197

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all FOXO4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from human FOXO4.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

forkhead box O4 , AFX , AFX1 , MLLT7

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]

Database

Research Area

DATA IMAGES

Anti-FOXO4 (phospho Ser197) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50197

GTX50197 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50197 FOXO4 (phospho Ser197) antibody.

Anti-FOXO4 (phospho Ser197) antibody used in Western Blot (WB). GTX50197

GTX50197 WB Image

WB analysis of extracts from 293 cells untreated or treated with serum using GTX50197 FOXO4 (phospho Ser197) antibody.

Anti-FOXO4 (phospho Ser197) antibody used in IHC (Paraffin sections) (IHC-P). GTX50197

GTX50197 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50197 FOXO4 (phospho Ser197) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399