APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 120-370 of human FURIN (NP_002560.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
furin, paired basic amino acid cleaving enzyme , FUR , PACE , PCSK3 , SPC1
Cellular Localization
Cell membrane,Secreted,Endosome membrane
Background
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Database
Research Area
DATA IMAGES
|
GTX55628 ICC/IF Image
ICC/IF analysis of A549 cells using GTX55628 Furin antibody. Blue : DAPI Dilution : 1:150
|
|
GTX55628 WB Image
WB analysis of various sample lysates using GTX55628 Furin antibody. Dilution : 1:1000 Loading : 25μg per lane
|
|
GTX55628 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX55628 Furin antibody. Blue : DAPI Dilution : 1:150
|
|
GTX55628 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX55628 Furin antibody. Dilution : 1:100
|
|
GTX55628 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX55628 Furin antibody. Blue : DAPI Dilution : 1:150
|
REFERENCE
There are currently no references for Furin antibody (GTX55628). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Furin antibody (GTX55628). Be the first to share your experience with this product.