APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.3-1μg/ml |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89575-PEP).
Product Note
This antibody is expected to recognise both reported variants.
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-TLEDNEERMSRLSK, from the internal region of the protein sequence according to NP_000809.1; NP_001127838.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamate decarboxylase 2 , GAD65
Cellular Localization
Cytoplasm, cytosol , Cytoplasmic vesicle , Golgi apparatus membrane
Background
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
Database
Research Area
DATA IMAGES
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GTX89575 IHC-P Image
IHC-P analysis of human colon using GTX89575 GAD65 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 2.5μg/ml
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GTX89575 WB Image
WB analysis of human hippocampus lysate using GTX89575 GAD65 antibody, Internal. Dilution : 0.3μg/ml Loading : 35μg protein in RIPA buffer
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GTX89575 IHC-P Image
IHC-P analysis of human cerebellum using GTX89575 GAD65 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 2.5μg/ml
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REFERENCE
There are currently no references for GAD65 antibody, Internal (GTX89575). Be the first to share your publications with this product.
REVIEW
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