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GAPDH antibody [GA1R]

Anti-GAPDH antibody [GA1R] used in Western Blot (WB). GTX82560
Anti-GAPDH antibody [GA1R] used in Western Blot (WB). GTX82560

Cat. No. GTX82560

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GA1R

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA, IHC

Reactivity

 Human, Mouse, Rat, Rabbit, Hamster, Chicken, Saccharomyces cerevisiae, Bacteria
Package
100 μg ($399)
See all GAPDH products

APPLICATION

Application Note

Recommended Starting Dilutions:
WB (with ECL): Use at a dilution of 1:1000-10000
Immunostaining: Use at a dilution of 1:500 - 1:2000

For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test

Calculated MW

36 kDa. ( Note )

Product Note

Recognizes native and denatured forms of GAPDH (∼37kDa). Recognizes GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable.

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant GAPDH

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Cellular Localization

Cytoplasm, cytosol , Nucleus , Membrane , Cytoplasm, cytoskeleton

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody [GA1R] used in Western Blot (WB). GTX82560

GTX82560 WB Image

1:2000 (0.5μg/mL) Ab dilution used in WB of 5μg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6)

Anti-GAPDH antibody [GA1R] used in Western Blot (WB). GTX82560

GTX82560 WB Image

WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)

REFERENCE

Application Reference

Rowe AA et al. EBioMedicine 2022; 85 : 104314 Long-term progression of retinal degeneration in a preclinical model of CLN7 Batten disease as a baseline for testing clinical therapeutics.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations