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GAPDH antibody [GT239] - VetSignal

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013
Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013

Cat. No. GTX635013

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT239

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Cat, Dog
Package
100 μl ($399)
See all GAPDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Human, Mouse, Rat, Zebrafish, Rabbit, Sheep, Bovine, Chicken, Guinea pig, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Recombinant protein corresponding to dog GAPDH.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888497

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase

Database

DATA IMAGES

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat kidney.
GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog tongue.
GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635013

GTX635013 ICC/IF Image

GAPDH antibody [GT239] detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAPDH stained by GAPDH antibody [GT239] (GTX635013) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-GAPDH antibody [GT239] - VetSignal used in IHC (Paraffin sections) (IHC-P). GTX635013

GTX635013 IHC-P Image

GAPDH antibody detects GAPDH protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded cat liver.
GAPDH stained by GAPDH antibody (GTX635013) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013

GTX635013 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GAPDH antibody [GT239] - VetSignal used in Western Blot (WB). GTX635013

GTX635013 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody (GTX635013) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations