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GAPDH antibody [HL2062]

Recombinant antibody
Anti-GAPDH antibody [HL2062] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637966
Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966
Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966
Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966
Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966
Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966
Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966
Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966

Cat. No. GTX637966

Reference 1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2062

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Zebrafish, Rabbit, Cat, Dog, Hamster, African green monkey, E. coli
Package
100 μl ($399),
25 μl ($169)
See all GAPDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:50000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human GAPDH

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glyceraldehyde-3-phosphate dehydrogenase , G3PD , GAPD , HEL-S-162eP

Cellular Localization

Cytoplasm, cytosol,Nucleus,Cytoplasm, perinuclear region,Membrane,Cytoplasm, cytoskeleton

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Database

Research Area

DATA IMAGES

Anti-GAPDH antibody [HL2062] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637966

GTX637966 ICC/IF Image

GAPDH antibody [HL2062] detects GAPDH protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GAPDH stained by GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966

GTX637966 WB Image

E. Coli (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966

GTX637966 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966

GTX637966 WB Image

Various whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966

GTX637966 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966

GTX637966 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966

GTX637966 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GAPDH antibody [HL2062] used in Western Blot (WB). GTX637966

GTX637966 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GAPDH antibody [HL2062] (GTX637966) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

JH Kim et al. J Neurosci 2024; : e1806232024 GABAergic/glycinergic and glutamatergic neurons mediate distinct neurodevelopmental phenotypes of STXBP1 encephalopathy
Application : WB
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Package List Price ($)
$ 399
$ 169
Custom Conjugations