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Gli1 antibody

Anti-Gli1 antibody used in Western Blot (WB). GTX124274
Anti-Gli1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124274
Anti-Gli1 antibody used in Western Blot (WB). GTX124274
Anti-Gli1 antibody used in Western Blot (WB). GTX124274
Anti-Gli1 antibody used in Western Blot (WB). GTX124274

Cat. No. GTX124274

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ChIP-seq

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Gli1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
ChIP-seq Assay dependent
Not tested in other applications.

Calculated MW

119 kDa. ( Note )

Positive Control

RMS13

Predict Reactivity

Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse Gli1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174802

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GLI-Kruppel family member GLI1 , AV235269 , Zfp-5 , Zfp5

Cellular Localization

Cytoplasm , Nucleus

Background

May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.

Database

Research Area

DATA IMAGES

Anti-Gli1 antibody used in Western Blot (WB). GTX124274

GTX124274 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Gli1 antibody (GTX124274) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Gli1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124274

GTX124274 ICC/IF Image

Gli1 antibody detects Gli1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: RMS-13 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Gli1 stained by Gli1 antibody (GTX124274) diluted at 1:5000.

Anti-Gli1 antibody used in Western Blot (WB). GTX124274

GTX124274 WB Image

The data was published in the journal Cancer Cell Int in 2017. PMID: 29225516

Anti-Gli1 antibody used in Western Blot (WB). GTX124274

GTX124274 WB Image

The data was published in the journal Cancer Cell Int in 2017. PMID: 29225516

Anti-Gli1 antibody used in Western Blot (WB). GTX124274

GTX124274 WB Image

The data was published in the journal Cancer Cell Int in 2017. PMID: 29225516

REFERENCE

Application Reference

Fritzi Ott et al. Cells 2022; 11 (10) Hepatic Hedgehog Signaling Participates in the Crosstalk between Liver and Adipose Tissue in Mice by Regulating FGF21.
Application : ChIP-seq
Reactivity : Mouse
Wang K et al. Cancer Cell Int 2017; 17 : 117 hedgehog/gli1 signaling pathway regulates mgmt expression and chemoresistance to * in human glioblastoma.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169